Recombinant e.coli ansA eiwit

AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB) . AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E. coli ansA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytoplasmic L-asparaginase I, ECK1765, JW1756

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

39.3 kDa (358aa) confirmed by MALDI-TOF

AnsA, Cytoplasmic L-asparaginase I, ECK1765, JW1756, ATGP1077-10 µg, ATGP1077-20 µg, ATGP1077-50 µg, ATGP1077-100 µg, ATGP1077-250 µg, ATGP1077-500 µg, ATGP1077-1 mg, ATGP1077-10, ATGP1077-20, ATGP1077-50, ATGP1077-100, ATGP1077-250, ATGP1077-500, ATGP1077-1

Cantor JR., et al. (2009) Biochemistry. 48 (46) :11026-31. ; ; Weidle uH., et al. (2009) Anticancer Res. 29 (4) :951-63.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Enzymes & Proteases

NP_416281

P0A962

E.coli

MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD