Recombinant E.coli Beta-lactamase-eiwit

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is >700 units/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C

AmpC, ampA, Cephalosporinase

E.coli

E.coli

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is >700unit/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C

41.8 kDa (379aa) confirmed by MALDI-TOF

B-lactamase, ATGP3575-10 µg, ATGP3575-20 µg, ATGP3575-50 µg, ATGP3575-100 µg, ATGP3575-250 µg, ATGP3575-500 µg, ATGP3575-1 mg, ATGP3575-10, ATGP3575-20, ATGP3575-50, ATGP3575-100, ATGP3575-250, ATGP3575-500, ATGP3575-1

Zhao Z., et al. (2015) Cancer Lett. 358 (1) :17-26.; ; Martin I., et al. (2014) Cell. 157 (2) :472-85.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_418574

P00811

E.coli

APQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ