Recombinant E.coli Carbonische anhydrase 2/CA2-eiwit

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3- + H+) . Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques.

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Carbonate dehydratase, CAN, ECK0125, JW0122, yadF

E.coli

E.coli

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 1,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

27.2 kDa (240aa) confirmed by MALDI-TOF

YadF, LAMP4, JW0122, GP110, ECK0125, CD68, Carbonic anhydrase2, Carbonic Anhydrase I, Carbonic anhydrase B., Carbonic anhydrase 1, Carbonate dehydratase I, Carbonate dehydratase, Car1, Car 1, CAN, CAI, CA1, CA I, CA 1, ATGP3240-10 µg, ATGP3240-20 µg, ATGP3240-50 µg, ATGP3240-100 µg, ATGP3240-250 µg, ATGP3240-500 µg, ATGP3240-1 mg, ATGP3240-010, ATGP3240-020, ATGP3240-050, ATGP3240-100, ATGP3240-250, ATGP3240-500, ATGP3240-01M

Lindskog S., et al : (1997) Pharmacol Ther.74 (1) :1-20.; ; Sawaya MR., et al (2006) J Biol Chem. 281 (11) :7546-55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Enzymes & Proteases

NP_414668

P61517

E.coli

MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK