Recombinant e.coli coaA eiwit

CoaA (pantothenate kinase) belongs to the prokaryotic pantothenate kinase family. Pantothenate kinase (PanK; CoaA) is the first enzyme in the Coenzyme A biosynthetic pathway. It phophorylates pantothenate (vitamin B5) to form 4'-phosphopantothenate. Regulation of pantothenate kinase activity by feedback inhibition is the critical factor controlling the intracellular CoA concentration. Recombinant E. coli coaA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pantothenate kinase, panK, rts, ts-9

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

38.9 kDa (340aa) confirmed by MALDI-TOF

CoaA, pantothenate kinase, panK, rts, ts-9, ATGP1395-10 µg, ATGP1395-20 µg, ATGP1395-50 µg, ATGP1395-100 µg, ATGP1395-250 µg, ATGP1395-500 µg, ATGP1395-1 mg, ATGP1395-10, ATGP1395-20, ATGP1395-50, ATGP1395-100, ATGP1395-250, ATGP1395-500, ATGP1395-1

Song W.-J., et al. (1992) J.Bacteriol. 174:6411-6417; ; Song W.-J., et al. (1992) J.Bacteriol. 174:1705-1706

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Metabolism

NP_418405

P0A6I3

E.coli

MGSSHHHHHH SSGLVPRGSH MGSHMSIKEQ TLMTPYLQFD RNQWAALRDS VPMTLSEDEI ARLKGINEDL SLEEVAEIYL PLSRLLNFYI SSNLRRQAVL EQFLGTNGQR IPYIISIAGS VAVGKSTTAR VLQALLSRWP EHRRVELITT DGFLHPNQVL KERGLMKKKG FPESYDMHRL VKFVSDLKSG VPNVTAPVYS HLIYDVIPDG DKTVVQPDIL ILEGLNVLQS GMDYPHDPHH VFVSDFVDFS IYVDAPEDLL QTWYINRFLK FREGAFTDPD SYFHNYAKLT KEEAIKTAMT LWKEINWLNL KQNILPTRER ASLILTKSAN HAVEEVRLRK