Recombinant e.coli cysH-eiwit

CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3', 5'-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3'-phosphoadenylyl sulfate and thioredoxin. Recombinant E. coli cysH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphoadenosine phosphosulfate reductase

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.1 kDa (264aa) confirmed by MALDI-TOF

CysH, Phosphoadenosine phosphosulfate reductase, ATGP1041-10 µg, ATGP1041-20 µg, ATGP1041-50 µg, ATGP1041-100 µg, ATGP1041-250 µg, ATGP1041-500 µg, ATGP1041-1 mg, ATGP1041-10, ATGP1041-20, ATGP1041-50, ATGP1041-100, ATGP1041-250, ATGP1041-500, ATGP1041-1

Berendt u., et al. (1995) Eur. J. Biochem. 233 (1) : 347-56.; ; Krone F.A., et al. (1990) FEBS Lett. 260:6-9

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol

Redox Proteins

NP_417242

P17854

E.coli

MGSSHHHHHH SSGLVPRGSH MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG