Recombinant E.coli D-lactate dehydrogenase/LDHA-eiwit

Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D) -lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E. coli IdhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

NAD-dependent, JW1375, htpH, hslI, hslF, Fermentative D-lactate dehydrogenase, ECK1377

E.coli

E.coli

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

39.1 kDa (353aa) confirmed by MALDI-TOF

D-lactate dehydrogenase, ldhA, fermentative D-lactate dehydrogenase NAD-dependent, fermentative D-lactate dehydrogenase, NAD-dependent, ECK1377, hslF, hslI, htpH, JW1375, ATGP3236-10 µg, ATGP3236-20 µg, ATGP3236-50 µg, ATGP3236-100 µg, ATGP3236-250 µg, ATGP3236-500 µg, ATGP3236-1 mg, ATGP3236-10, ATGP3236-20, ATGP3236-50, ATGP3236-100, ATGP3236-250, ATGP3236-500, ATGP3236-1

Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1) : 13-22.; ; Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11) : 25026-25037.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100 mM NaCl

Redox Proteins

NP_415898.1

P52643

E.coli

MKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV