Recombinant E.coli dsbE-eiwit

DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography.

Thiol:disulfide interchange protein dsbE, CcmG, yejQ, Cytochrome c biogenesis protein ccmG, ECK2187, Periplasmic thioredoxin of cytochrome c type biogenesis, JW2183

E.coli

E.coli

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18.1 kDa (161aa) confirmed by MALDI-TOF

DsbE, Thiol:disulfide interchange protein dsbE, CcmG, yejQ, Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG..ECK2187, Periplasmic thioredoxin of cytochrome c type biogenesis, JW2183., ATGP0283-10 µg, ATGP0283-20 µg, ATGP0283-50 µg, ATGP0283-100 µg, ATGP0283-250 µg, ATGP0283-500 µg, ATGP0283-1 mg, ATGP0283-10, ATGP0283-20, ATGP0283-50, ATGP0283-100, ATGP0283-250, ATGP0283-500, ATGP0283-1

Reid E., et al. (2001) . Biochem J. 355 (1) :51-8.; ; Li Q., et al. (2001) . Biol Chem. 382 (12) :1679-86;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA 10% glycerol

Heat Shock Proteins

NP_416699

P0AA86

E.coli

MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q