Recombinant e.coli fimC-eiwit

The 23 kDa two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages. Recombinant E. coli FIMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Periplasmic chaperone required for type 1 fimbriae, Periplasmic chaperone, required for type 1 fimbriae, ECK4307, JW4279

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25 kDa (226aa) confirmed by MALDI-TOF

FimC, Periplasmic chaperone required for type 1 fimbriae, Periplasmic chaperone, required for type 1 fimbriae, ECK4307, JW4279, ATGP0931-10 µg, ATGP0931-20 µg, ATGP0931-50 µg, ATGP0931-100 µg, ATGP0931-250 µg, ATGP0931-500 µg, ATGP0931-1 mg, ATGP0931-10, ATGP0931-20, ATGP0931-50, ATGP0931-100, ATGP0931-250, ATGP0931-500, ATGP0931-1

Hermanns u., et al. (2000) Biochemistry. 39 (38) :11564-70.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Heat Shock Proteins

NP_418736

P31697

E.coli

GVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME