Recombinant e.coli-glpe-eiwit

Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. Recombinant E. coli glpE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Thiosulfate:cyanide sulfurtransferase (rhodanese), ECK3411, JW3388

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

14.5 kDa (131aa) confirmed by MALDI-TOF

GlpE, Thiosulfate:cyanide sulfurtransferase (rhodanese), ECK3411, JW3388, ATGP2144-10 µg, ATGP2144-20 µg, ATGP2144-50 µg, ATGP2144-100 µg, ATGP2144-250 µg, ATGP2144-500 µg, ATGP2144-1 mg, ATGP2144-10, ATGP2144-20, ATGP2144-50, ATGP2144-100, ATGP2144-250, ATGP2144-500, ATGP2144-1

Li H., et al. (2011) Biomol NMR Assign. 5 (1) :97-9; ; Ray WK., et al. (2000) J Bacteriol. 182 (8) :2277-84.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_417883

P0A6V5

E.coli

MDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A