Recombinant e.coli glsA1-eiwit

GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+. Recombinant E. coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutaminase1, ybaS

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.0 kDa (330aa) confirmed by MALDI-TOF

GlsA1, Glutaminase1, ybaS, ATGP1053-10 µg, ATGP1053-20 µg, ATGP1053-50 µg, ATGP1053-100 µg, ATGP1053-250 µg, ATGP1053-500 µg, ATGP1053-1 mg, ATGP1053-10, ATGP1053-20, ATGP1053-50, ATGP1053-100, ATGP1053-250, ATGP1053-500, ATGP1053-1

Brown G., et al. (2008) Biochemistry. 47 (21) :5724-35. ; ; Nandakumar R., et al. (2003) J. Mol. Catal. B: Enzymes. 23:87-100.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Enzymes & Proteases

NP_415018.1

P77454

E.coli

MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG