Recombinant e.coli gor eiwit

Gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond. Recombinant E. coli gor protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 60 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

Glutathione oxidoreductase, ECK3485, gorA, JW3467

E.coli

E.coli

His-Tag

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 60unit/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

51.2 kDa (473aa) confirmed by MALDI-TOF

Gor, Glutathione oxidoreductase, ECK3485, gorA, JW3467, ATGP1423-10 µg, ATGP1423-20 µg, ATGP1423-50 µg, ATGP1423-100 µg, ATGP1423-250 µg, ATGP1423-500 µg, ATGP1423-1 mg, ATGP1423-10, ATGP1423-20, ATGP1423-50, ATGP1423-100, ATGP1423-250, ATGP1423-500, ATGP1423-1

Staal G.E. et al. (1969) Biochim. Biophys. Acta 185: 63-69.; ; Stoll V.S.. et al. (1997) Biochemistry 36: 6437-6447.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Enzymes & Proteases

NP_417957

P06715

E.coli

MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR