Recombinant e.coli grxB eiwit

GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E. coli grxB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26.5 kDa (235aa) confirmed by MALDI-TOF

GrxB, glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2, ATGP1040-10 µg, ATGP1040-20 µg, ATGP1040-50 µg, ATGP1040-100 µg, ATGP1040-250 µg, ATGP1040-500 µg, ATGP1040-1 mg, ATGP1040-10, ATGP1040-20, ATGP1040-50, ATGP1040-100, ATGP1040-250, ATGP1040-500, ATGP1040-1

Holmgren A, et al. (2004) Antioxid. Redox. Signal. 6 (1) : 63-74.; ; Lopez-Maury L, et al. (2009) J Bacteriol. 191 (11) :3534-43.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Redox Proteins

NP_415582

P0AC59

E.coli

MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI