Recombinant E.coli idnK-eiwit

D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. Recombinant E. coli idnk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

D-gluconate kinase thermosensitive, D-gluconate kinase, thermosensitive, ECK4261, gntV, JW4225

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.4 kDa (210aa) confirmed by MALDI-TOF

Idnk, D-gluconate kinase thermosensitive, D-gluconate kinase, thermosensitive, ECK4261, gntV, JW4225, ATGP2833-10 µg, ATGP2833-20 µg, ATGP2833-50 µg, ATGP2833-100 µg, ATGP2833-250 µg, ATGP2833-500 µg, ATGP2833-1 mg, ATGP2833-10, ATGP2833-20, ATGP2833-50, ATGP2833-100, ATGP2833-250, ATGP2833-500, ATGP2833-1

Coppo P., et al. (2006) J Haematol. 134: 171-179.; ; Zheng X., et al. (2006) BMC cancer. 7: 262.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_418689

P39208

E.coli

MAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE