Recombinant E.coli L-lactate dehydrogenase/LLDD-eiwit

LldD, also known as L-lactate dehydrogenase, is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD) . Recombinant E. coli lldD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

L-lactate dehydrogenase, ECK3595, JW3580, lct, lctD

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.3 kDa (420aa) confirmed by MALDI-TOF

LldD, L-lactate dehydrogenase, ECK3595, JW3580, lct, lctD, ATGP1563-10 µg, ATGP1563-20 µg, ATGP1563-50 µg, ATGP1563-100 µg, ATGP1563-250 µg, ATGP1563-500 µg, ATGP1563-1 mg, ATGP1563-10, ATGP1563-20, ATGP1563-50, ATGP1563-100, ATGP1563-250, ATGP1563-500, ATGP1563-1

Ogura Y., et al. (2009) Proc. Natl. Acad. Sci. u.S.A. 106:17939-17944

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_418062

P33232

E.coli

MGSSHHHHHH SSGLVPRGSH MGSHMIISAA SDYRAAAQRI LPPFLFHYMD GGAYSEYTLR RNVEDLSEVA LRQRILKNMS DLSLETTLFN EKLSMPVALA PVGLCGMYAR RGEVQAAKAA DAHGIPFTLS TVSVCPIEEV APAIKRPMWF QLYVLRDRGF MRNALERAKA AGCSTLVFTV DMPTPGARYR DAHSGMSGPN AAMRRYLQAV THPQWAWDVG LNGRPHDLGN ISAYLGKPTG LEDYIGWLGN NFDPSISWKD LEWIRDFWDG PMVIKGILDP EDARDAVRFG ADGIVVSNHG GRQLDGVLSS ARALPAIADA VKGDIAILAD SGIRNGLDVV RMIALGADTV LLGRAFLYAL ATAGQAGVAN LLNLIEKEMK VAMTLTGAKS ISEITQDSLV QGLGKELPAA LAPMAKGNAA