Recombinant E.coli Maltose Bindeiwit/MBP-eiwit

Cytoplasmic maltose-binding protein, also known as MBP, is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant E. coli MBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ZCSL3, MMBP, MBP, Maltose binding protein, Maltodextrin-binding protein, malJ, malE, JW3994, JJJ3, Escherichia coli MBP, ECK4026, E.coli MBP, DPH4, Cytoplasmic maltose-binding protein

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

44.9 kDa (410aa) confirmed by MALDI-TOF

MBP, Cytoplasmic maltose-binding protein, DPH4, JJJ3, ZCSL3, ATGP2171-10 µg, ATGP2171-20 µg, ATGP2171-50 µg, ATGP2171-100 µg, ATGP2171-250 µg, ATGP2171-500 µg, ATGP2171-1 mg, ATGP2171-10, ATGP2171-20, ATGP2171-50, ATGP2171-100, ATGP2171-250, ATGP2171-500, ATGP2171-1

Fox JD., et al. (2001) Protein Sci. 10 (3) :622-30; ; Riggs P., et al. (2000) Mol Biotechnol. 15:51-63.

Moree B, et al. Protein Conformational Changes Are Detected and Resolved Site Specifically by Second-Harmonic Generation. (Biophys J. 2015) {https://pubmed.ncbi.nlm.nih.gov/26287632/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Fusion Tag & Detection

NP_418458.1

P0AEX9

E.coli

MKIEEGK LVIWINGDKG YNGLAEVGKK FEKDTGIKVT VEHPDKLEEK FPQVAATGDG PDIIFWAHDR FGGYAQSGLL AEITPDKAFQ DKLYPFTWDA VRYNGKLIAY PIAVEALSLI YNKDLLPNPP KTWEEIPALD KELKAKGKSA LMFNLQEPYF TWPLIAADGG YAFKYENGKY DIKDVGVDNA GAKAGLTFLV DLIKNKHMNA DTDYSIAEAA FNKGETAMTI NGPWAWSNID TSKVNYGVTV LPTFKGQPSK PFVGVLSAGI NAASPNKELA KEFLENYLLT DEGLEAVNKD KPLGAVALKS YEEELAKDPR IAATMENAQK GEIMPNIPQM SAFWYAVRTA VINAASGRQT VDEALKDAQT