Recombinant E.coli Maltose Bindeiwit/MBP-eiwit

MBP also as known as Maltose Binding Protein is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant MBP was expressed in E. coli and purified by conventional chromatography techniques.

ZCSL3, MMBP, MBP, Maltose binding protein, Maltodextrin-binding protein, malJ, malE, JW3994, JJJ3, Escherichia coli MBP, ECK4026, E.coli MBP, DPH4, Cytoplasmic maltose-binding protein

E.coli

E.coli

Non-Tagged

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

40.8 kDa (371aa)

MBP, ECK4026, JW3994, malJ, ATGP3346-10 µg, ATGP3346-20 µg, ATGP3346-50 µg, ATGP3346-100 µg, ATGP3346-250 µg, ATGP3346-500 µg, ATGP3346-1 mg, ATGP3346-10, ATGP3346-20, ATGP3346-50, ATGP3346-100, ATGP3346-250, ATGP3346-500, ATGP3346-1

Fox JD., et al. (2001) Protein Sci. 10 (3) : 622-30.; ; Riggs P., et al. (2000) Mol. Biotechnol. 15 (1) : 51-63.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Fusion Tag & Detection

NP_418458.1

P0AEX9

E.coli

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTRIT K