Recombinant e.coli Methionine Aminopeptidase/METAP-eiwit

Methionine aminopeptidases, also designated peptidase M proteins, are members of the M24 family of proteins. This protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of map contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Recombinant E. coli map protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methionine aminopeptidase., ECK0166, JW0163, pepM

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.5 kDa (284aa) confirmed by MALDI-TOF

Methionine Aminopeptidase, METAP, Methionine aminopeptidase., ECK0166, JW0163, pepM, ATGP1025-10 µg, ATGP1025-20 µg, ATGP1025-50 µg, ATGP1025-100 µg, ATGP1025-250 µg, ATGP1025-500 µg, ATGP1025-1 mg, ATGP1025-10, ATGP1025-20, ATGP1025-50, ATGP1025-100, ATGP1025-250, ATGP1025-500, ATGP1025-1

Bernier SG., et al. (2005) J Cell Biochem. 95 (6) :1191-203.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Enzymes & Proteases

NP_414710

P0AE18

E.coli

MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE