Recombinant e.coli mok eiwit

G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of u:G>u:A, although the biological role remains unclear. The enzyme uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E. coli mug protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, dug, ECK3058, JW3040, ygjF

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.1 kDa (191aa) confirmed by MALDI-TOF

Mug, G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, dug, ECK3058, JW3040, ygjF, ATGP2057-10 µg, ATGP2057-20 µg, ATGP2057-50 µg, ATGP2057-100 µg, ATGP2057-250 µg, ATGP2057-500 µg, ATGP2057-1 mg, ATGP2057-10, ATGP2057-20, ATGP2057-50, ATGP2057-100, ATGP2057-250, ATGP2057-500, ATGP2057-1

Lee HW., et al. (2010) J Biol Chem. 285 (53) :41483-90; ; Gallinari P., et al. (1996) Nature. 383 (6602) :735-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Enzymes & Proteases

NP_417540

P0A9H1

E.coli

MVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R