Recombinant e.coli msrA-eiwit

Peptide methionine sulfoxide reductase A, also known msrA, is an enzyme that catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. This protein could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Recombinant E. coli msrA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Peptide methionine sulfoxide reductase A, ECK4215, JW4178, pms, pmsR, peptide-methionine (S) -S-oxide reductase

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.4 kDa (232aa) confirmed by MALDI-TOF

MsrA, Peptide methionine sulfoxide reductase A, ECK4215, JW4178, pms, pmsR, peptide-methionine (S) -S-oxide reductase, ATGP1036-10 µg, ATGP1036-20 µg, ATGP1036-50 µg, ATGP1036-100 µg, ATGP1036-250 µg, ATGP1036-500 µg, ATGP1036-1 mg, ATGP1036-10, ATGP1036-20, ATGP1036-50, ATGP1036-100, ATGP1036-250, ATGP1036-500, ATGP1036-1

Kuschel L., et al. (1999) FEBS Letts.1:17-21.; ; Gabbita S., et al. (1999) J Neurochem. 4:1660-1666.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Redox Proteins

NP_418640

P0A744

E.coli

MGSSHHHHHH SSGLVPRGSH MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA