Recombinant e.coli msrB eiwit

MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E. coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes. Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methionine sulfoxide reductase B, ECK1776, JW1767, yeaA

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.6 kDa (157aa) confirmed by MALDI-TOF

MsrB, Methionine sulfoxide reductase B, ECK1776, JW1767, yeaA, ATGP1026-10 µg, ATGP1026-20 µg, ATGP1026-50 µg, ATGP1026-100 µg, ATGP1026-250 µg, ATGP1026-500 µg, ATGP1026-1 mg, ATGP1026-10, ATGP1026-20, ATGP1026-50, ATGP1026-100, ATGP1026-250, ATGP1026-500, ATGP1026-1

Zheung D. et al. (2003) J. Biomol. NMR. 27:183-184; ; Weissbach H. et al. (2002) Arch.Biochem.Biophys. 397: 172-178

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Signal Transduction

NP_416292

P0A746

E.coli

MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFY EPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING