Recombinant e.coli mutM-eiwit

MutM, also known as formamidopyrimidine DNA glycosylase, is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA. This protein is nonredundant and required to rapidly remove its substrate lesions on the chromosome. In addition, it also repaired a significant portion of the lesions recognized by Endo III, suggesting that it plays a prominent role in the global repair of both purine damage and pyrimidine damage in vivo. Recombinant E. coli mutM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Formamidopyrimidine-DNA glycosylase, fpg

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

32.4 kDa (289aa) confirmed by MALDI-TOF

MutM, ATGP1448-10 µg, ATGP1448-20 µg, ATGP1448-50 µg, ATGP1448-100 µg, ATGP1448-250 µg, ATGP1448-500 µg, ATGP1448-1 mg, ATGP1448-10, ATGP1448-20, ATGP1448-50, ATGP1448-100, ATGP1448-250, ATGP1448-500, ATGP1448-1

Serre L. et al. (2002) EMBO J. 21:2854-2865; ; Schalow BJ. et al. (2011) J Mol Biol, 410:183-193;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Enzymes & Proteases

NP_418092

P05523

E.coli

MPELPEVETS RRGIEPHLVG ATILHAVVRN GRLRWPVSEE IYRLSDQPVL SVQRRAKYLL LELPEGWIII HLGMSGSLRI LPEELPPEKH DHVDLVMSNG KVLRYTDPRR FGAWLWTKEL EGHNVLTHLG PEPLSDDFNG EYLHQKCAKK KTAIKPWLMD NKLVVGVGNI YASESLFAAG IHPDRLASSL SLAECELLAR VIKAVLLRSI EQGGTTLKDF LQSDGKPGYF AQELQVYGRK GEPCRVCGTP IVATKHAQRA TFYCRQCQK