Recombinant e.coli nanana-eiwit

NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E. coli nanA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

N-acetylneuraminate lyase, npl

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

34.7 kDa (317aa) confirmed by MALDI-TOF

NanA, N-acetylneuraminate lyase, npl, ATGP1032-10 µg, ATGP1032-20 µg, ATGP1032-50 µg, ATGP1032-100 µg, ATGP1032-250 µg, ATGP1032-500 µg, ATGP1032-1 mg, ATGP1032-10, ATGP1032-20, ATGP1032-50, ATGP1032-100, ATGP1032-250, ATGP1032-500, ATGP1032-1

Aisaka K., et al. (1991) Biochem. J. 276:541-546; ; Izard T., et al. (1994) Structure 2:361-369

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Enzymes & Proteases

NP_417692

P0A6L4

E.coli

MGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEA KGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPG VGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTG VFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG