Recombinant e.coli ndk-eiwit

Ndk, also known as Nucleoside diphosphate kinase, is an enzyme that catalyzes the exchange of phosphate groups between different nucleoside diphosphates. This protein activity maintains an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when GTP produced in the citric acid (Krebs) cycle is converted to ATP. Recombinant E. coli ndk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleoside diphosphate kinase, ECK2514, JW2502

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18 kDa (167aa) confirmed by MALDI-TOF

Ndk, Nucleoside diphosphate kinase, ECK2514, JW2502, ATGP1424-10 µg, ATGP1424-20 µg, ATGP1424-50 µg, ATGP1424-100 µg, ATGP1424-250 µg, ATGP1424-500 µg, ATGP1424-1 mg, ATGP1424-10, ATGP1424-20, ATGP1424-50, ATGP1424-100, ATGP1424-250, ATGP1424-500, ATGP1424-1

Lee B. et al. (2009) FEBS Lett. 583:3291-3295; ; Kihara A. et al. (2011) Biosci Biotechnol Biochem, 75:1740-1745

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Enzymes & Proteases

NP_417013

P0A763

E.coli

MAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR