Recombinant E.coli nfsB-eiwit

NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl) -2, 4-dinitrobenzamide) . The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E. coli NFNB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Oxygen-insensitive NAD (P) H nitroreductase, Dihydropteridine reductase, FMN-dependent nitroreductase, dprA, nfnB

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26.0 kDa (237aa) confirmed by MALDI-TOF

NFNB, dihydropteridine reductase NAD (P) H-dependent oxygen-insensitive, dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD (P) H-dependent, oxygen-insensitive, ATGP0648-10 µg, ATGP0648-20 µg, ATGP0648-50 µg, ATGP0648-100 µg, ATGP0648-250 µg, ATGP0648-500 µg, ATGP0648-1 mg, ATGP0648-10, ATGP0648-20, ATGP0648-50, ATGP0648-100, ATGP0648-250, ATGP0648-500, ATGP0648-1

Jarrom D., et al. (2009) Biochemistry. 48 (32) :7665-72. ; ; Singleton DC., et al. (2007) Cancer Gene Ther. 14 (12) :953-67.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM Nacl, 1mM DTT, 10% glycerol

Redox Proteins

NP_415110

P38489

E.coli

MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV