Recombinant e.coli nth-eiwit

Endonuclease III, also known as nth, is a DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Recombinant E. coli nth protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Endonuclease III, b1633, JW1625, DNA- (apurinic or apyrimidinic site) lyase, Endonuclease-III

E.coli

E.coli

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.7 kDa (231aa) confirmed by MALDI-TOF

Nth, Endonuclease III, b1633, JW1625, DNA- (apurinic or apyrimidinic site) lyase, Endonuclease-III, ATGP1052-10 µg, ATGP1052-20 µg, ATGP1052-50 µg, ATGP1052-100 µg, ATGP1052-250 µg, ATGP1052-500 µg, ATGP1052-1 mg, ATGP1052-10, ATGP1052-20, ATGP1052-50, ATGP1052-100, ATGP1052-250, ATGP1052-500, ATGP1052-1

Cunningham R.P., et al. (1994) Acad. Sci. 726:215-222; ; Asahara H., et al. (1989) Biochemistry. 28:4444-4449

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF

Enzymes & Proteases

NP_416150

P0AB83

E.coli

MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I