Recombinant e.coli phrB-eiwit

Deoxyribodipyrimidine photolyase, also known as phrB, is involved in repair of uV radiation-induced DNA damage. This protein catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Recombinant E. coli phrB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Deoxyribodipyrimidine photolyase, ECK0697, JW0698, phrB

E.coli

E.coli

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

56.1 kDa (495aa)

PhrB, Deoxyribodipyrimidine photolyase, ECK0697, JW0698, phrB, ATGP2044-10 µg, ATGP2044-20 µg, ATGP2044-50 µg, ATGP2044-100 µg, ATGP2044-250 µg, ATGP2044-500 µg, ATGP2044-1 mg, ATGP2044-10, ATGP2044-20, ATGP2044-50, ATGP2044-100, ATGP2044-250, ATGP2044-500, ATGP2044-1

Byrdin M., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:8676-8681; ; Weber S., et al. (2005) Biochim. Biophys. Acta. 1707:1-23;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT

Enzymes & Proteases

NP_415236.1

P00914

E.coli

MTTHLVW FRQDLRLHDN LALAAACRNS SARVLALYIA TPRQWATHNM SPRQAELINA QLNGLQIALA EKGIPLLFRE VDDFVASVEI VKQVCAENSV THLFYNYQYE VNERARDVEV ERALRNVVCE GFDDSVILPP GAVMTGNHEM YKVFTPFKNA WLKRLREGMP ECVAAPKVRS SGSIEPSPSI TLNYPRQSFD TAHFPVEEKA AIAQLRQFCQ NGAGEYEQQR DFPAVEGTSR LSASLATGGL SPRQCLHRLL AEQPQALDGG AGSVWLNELI WREFYRHLIT YHPSLCKHRP FIAWTDRVQW QSNPAHLQAW QEGKTGYPIV DAAMRQLNST GWMHNRLRMI TASFLVKDLL IDWREGERYF MSQLIDGDLA ANNGGWQWAA STGTDAAPYF RIFNPTTQGE KFDHEGEFIR QWLPELRDVP GKVVHEPWKW AQKAGVTLDY PQPIVEHKEA RVQTLAAYEA ARKGK