Recombinant e.coli ppa-eiwit

Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E. coli ppa protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Inorganic pyrophosphatase, ECK4222, JW4185

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

21.9 kDa (196aa) confirmed by MALDI-TOF

Ppa, inorganic pyrophosphatase, ECK4222, JW4185, ATGP1132-10 µg, ATGP1132-20 µg, ATGP1132-50 µg, ATGP1132-100 µg, ATGP1132-250 µg, ATGP1132-500 µg, ATGP1132-1 mg, ATGP1132-10, ATGP1132-20, ATGP1132-50, ATGP1132-100, ATGP1132-250, ATGP1132-500, ATGP1132-1

Harold FM., et al (1966) Bacteriol Rev 30 (4) : 772-94.; ; Carman GM., et al (2006) Trends Biochem. Sci. 31 (12) : 694-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Signal Transduction

NP_418647

P0A7A9

E.coli

MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK