Recombinant e.coli pykF-eiwit

PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E. coli pykF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pyruvate kinase I, ECK1672, JW1666

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

53.3 kDa (494aa)

PykF, pyruvate kinase I, ECK1672, JW1666, ATGP1503-10 µg, ATGP1503-20 µg, ATGP1503-50 µg, ATGP1503-100 µg, ATGP1503-250 µg, ATGP1503-500 µg, ATGP1503-1 mg, ATGP1503-10, ATGP1503-20, ATGP1503-50, ATGP1503-100, ATGP1503-250, ATGP1503-500, ATGP1503-1

Liapounova, Na, et al. (2006) Eukaryotic cell 5 (12) : 2138-46, ; ; Valentini G., et al. (2000) J. Biol. Chem. 275:18145-18152

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_416191

P0AD61

E.coli

MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL