Recombinant e.coli rnhA-eiwit

RnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e. coli rnhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20 kDa (178aa) confirmed by MALDI-TOF

RnhA, Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin, ATGP1173-10 µg, ATGP1173-20 µg, ATGP1173-50 µg, ATGP1173-100 µg, ATGP1173-250 µg, ATGP1173-500 µg, ATGP1173-1 mg, ATGP1173-10, ATGP1173-20, ATGP1173-50, ATGP1173-100, ATGP1173-250, ATGP1173-500, ATGP1173-1

Nowotny M., et al. (2007) Mol Cell. 28 (2) :264-76.; ; Ten Asbroek AL., et al. (2002) Eur J Biochem. 269 (2) :583-92.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Enzymes & Proteases

NP_414750

P0A7Y4

E.coli

MLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV