Recombinant E.coli skp eiwit

The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane. Recombinant E. coli Skp, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Chaperone protein skp, Periplasmic chaperone, ompH, DNA binding 17 kDa protein, ECK0177, Histone like protein HLP 1, hlpA, JW0173, Seventeen kilodalton protein

E.coli

E.coli

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.9 kDa (162aa) confirmed by MALDI-TOF

Skp, Chaperone protein skp, Chaperone protein skp, periplasmic chaperone, ompH, DNA binding 17 kDa protein, ECK0177, Histone like protein HLP 1, hlpA, JW0173, Seventeen kilodalton protein, ATGP0589-10 µg, ATGP0589-20 µg, ATGP0589-50 µg, ATGP0589-100 µg, ATGP0589-250 µg, ATGP0589-500 µg, ATGP0589-1 mg, ATGP0589-10, ATGP0589-20, ATGP0589-50, ATGP0589-100, ATGP0589-250, ATGP0589-500, ATGP0589-1

Korndorfer IP., et al. (2004) Nat Struct Mol Biol. 11 (10) :1015-20; ; Walton TA., et al. (2004) Mol Cell. 15 (3) :367-74.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Heat Shock Proteins

NP_414720

P0AEU7

E.coli

ADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK