Recombinant E.coli surA-eiwit

SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E. coli in stationary phase and required for pilus biogenesis. Recombinant E. coli SurA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Chaperone surA, PPIase surA, Rotamase surA, Survival protein A

E.coli

E.coli

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

47.3 kDa (429aa) confirmed by MALDI-TOF

SurA, Chaperone surA, PPIase surA, Rotamase surA, Survival protein A, Chaperone surA, ATGP0664-10 µg, ATGP0664-20 µg, ATGP0664-50 µg, ATGP0664-100 µg, ATGP0664-250 µg, ATGP0664-500 µg, ATGP0664-1 mg, ATGP0664-10, ATGP0664-20, ATGP0664-50, ATGP0664-100, ATGP0664-250, ATGP0664-500, ATGP0664-1

Virlogeux-Payant I., et al (2009) Microbiology. 155 (Pt 5) :1613-22; ; Kolter R., et al. (1996) J Bacteriol. 178 (6) :1770-3.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Heat Shock Proteins

NP_414595

P0ABZ6

E.coli

MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN