Recombinant E.coli tnaA-eiwit

TnaA, also known as Tryptophanase/L-cysteine desulfhydrase. Tryptophanase is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are L-tryptophan and H2O, whereas its 3 products are indole, pyruvate, and NH3. Recombinant E. coli tnaA, fused to His-tag at N-terminus, was expressed in E. coli and purified using conventional chromatography techniques.

Tryptophanase/L-cysteine desulfhydrase PLP-dependent, Tryptophanase/L-cysteine desulfhydrase, PLP-dependent, Tryptophanase

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

55.2 kDa (494aa)

TnaA, ATGP2963-10 µg, ATGP2963-20 µg, ATGP2963-50 µg, ATGP2963-100 µg, ATGP2963-250 µg, ATGP2963-500 µg, ATGP2963-1 mg, ATGP2963-10, ATGP2963-20, ATGP2963-50, ATGP2963-100, ATGP2963-250, ATGP2963-500, ATGP2963-1

Tokushige M., et al. (1989) Biochimie 71:711-720

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_418164

P0A853

E.coli

MENFKHL PEPFRIRVIE PVKRTTRAYR EEAIIKSGMN PFLLDSEDVF IDLLTDSGTG AVTQSMQAAM MRGDEAYSGS RSYYALAESV KNIFGYQYTI PTHQGRGAEQ IYIPVLIKKR EQEKGLDRSK MVAFSNYFFD TTQGHSQING CTVRNVYIKE AFDTGVRYDF KGNFDLEGLE RGIEEVGPNN VPYIVATITS NSAGGQPVSL ANLKAMYSIA KKYDIPVVMD SARFAENAYF IKQREAEYKD WTIEQITRET YKYADMLAMS AKKDAMVPMG GLLCMKDDSF FDVYTECRTL CVVQEGFPTY GGLEGGAMER LAVGLYDGMN LDWLAYRIAQ VQYLVDGLEE IGVVCQQAGG HAAFVDAGKL LPHIPADQFP AQALACELYK VAGIRAVEIG SFLLGRDPKT GKQLPCPAEL LRLTIPRATY TQTHMDFIIE AFKHVKENAA NIKGLTFTYE PKVLRHFTAK LKEV