Recombinant E.coli txA-eiwit

Thioredoxin 1 (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant E. coli TXN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Thioredoxin 1, TrxA1, TrxA2

E.coli

E.coli

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

Specific activity is >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

12.8 kDa (117aa) confirmed by MALDI-TOF

Thioredoxin1, dasC, fipA, tsnC, ATGP0549-10 µg, ATGP0549-20 µg, ATGP0549-50 µg, ATGP0549-100 µg, ATGP0549-250 µg, ATGP0549-500 µg, ATGP0549-1 mg, ATGP0549-010, ATGP0549-020, ATGP0549-050, ATGP0549-100, ATGP0549-250, ATGP0549-500, ATGP0549-01M

Holmgren A., et al. (1989) J Biol Chem. 264 (24) :13963-6. ; ; Hacquot JP., et al. (1990) Biochem Biophys Res Commun. 173 (3) :1375-81.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Redox Proteins

ACB04810

W8TGE9

E.coli

MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLA