Recombinant E.coli txB eiwit

TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, FAD/NAD (P) -binding, TRXR

E.coli

E.coli

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 10unit/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

34.6 kDa (321aa) confirmed by MALDI-TOF

TRXB, Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR., ATGP0540-10 µg, ATGP0540-20 µg, ATGP0540-50 µg, ATGP0540-100 µg, ATGP0540-250 µg, ATGP0540-500 µg, ATGP0540-1 mg, ATGP0540-10, ATGP0540-20, ATGP0540-50, ATGP0540-100, ATGP0540-250, ATGP0540-500, ATGP0540-1

Faulkner MJ., et al. (2008) Proc Natl Acad Sci u S A. 105 (18) :6735-40. ; ; Takahata M., et al. (2008) J Biochem. 143 (4) :467-73.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Redox Proteins

YP_003044110.1

P0A9P4

E.coli

MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K