Recombinant E.coliung-eiwit

Ung, also known as uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. uracil bases occur from cytosine deamination or misincorporation of duMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E. coli ung protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Uracil-DNA-glycosylase, ECK2578, JW2564, UDG, UNG1, UNG2, HIGM4

E.coli

E.coli

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.1 kDa (252aa) confirmed by MALDI-TOF

Ung, uracil-DNA-glycosylase, ECK2578, JW2564, ATGP2365-10 µg, ATGP2365-20 µg, ATGP2365-50 µg, ATGP2365-100 µg, ATGP2365-250 µg, ATGP2365-500 µg, ATGP2365-1 mg, ATGP2365-10, ATGP2365-20, ATGP2365-50, ATGP2365-100, ATGP2365-250, ATGP2365-500, ATGP2365-1

Lindahl T. et al. (1977) J Biol Chem. 252 : 3286-3294.; ; Pearl, L. H. et al. (2000) Mutation research 460 : 165-181.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_417075

P12295

E.coli

MANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE