Recombinant GDNFR alfa-1/GFRA1-eiwit van rat

Gfra1, also known as GDNF family receptor alpha-1, is a member of the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI) -linked cell surface receptor for both Glial cell line-derived growth factor (GDNF), neurturin (NTN), and mediates activation of the RET tyrosine kinase receptor. This protein is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Recombinant rat Gfra1 protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

GDNF family receptor alpha-1, Gfra1, GFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, Retl1, Trnr1

Baculovirus

Rat

HIgG-His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

72.3 kDa (645aa)

GDNF family receptor alpha 1, GDNF family receptor alpha-1, GFR a1, GFR alpha 1, Gfra 1, Gfra1, Gfra-1, GFR-alpha-1, RET ligand 1, Retl 1, Retl1, TGF beta related neurotrophic factor receptor 1, TGF-beta-related neurotrophic factor receptor 1, Trnr 1, Trnr1, ATGP3579-10 µg, ATGP3579-20 µg, ATGP3579-50 µg, ATGP3579-100 µg, ATGP3579-250 µg, ATGP3579-500 µg, ATGP3579-1 mg, ATGP3579-010, ATGP3579-020, ATGP3579-050, ATGP3579-100, ATGP3579-250, ATGP3579-500, ATGP3579-01M

Gassei K. et al., (2009) Cell Tissue Res. 337:177-183.; ; Irala D. et al., (2016) Development. 143: 4224-4235.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_037091

Q62997

Rat

Asp25

DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLTS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRA VPFISDVFQQ VEHISKGNNC LDAAKACNLD DTCKKYRSAY ITPCTTSMSN EVCNRRKCHK ALRQFFDKVP AKHSYGMLFC SCRDIACTER RRQTIVPVCS YEERERPNCL SLQDSCKTNY ICRSRLADFF TNCQPESRSV SNCLKENYAD CLLAYSGLIG TVMTPNYVDS SSLSVAPWCD CSNSGNDLED CLKFLNFFKD NTCLKNAIQA FGNGSDVTMW QPAPPVQTTT ATTTTAFRVK NKPLGPAGSE NEIPTHVLPP CANLQAQKLK SNVSGSTHLC LSDSDFGKDG LAGASSLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH