Recombinant GLUT1 Antilichaam

Recognizes a protein of 45-55kDa, which is identified as GLUT1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut1 to Glut12. Glut1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut1 is expressed at variable levels in many human tissues. Overexpression has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative) .

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

P11166

Human

A portion of amino acids 203-305 from the human protein was used as the immunogen for this recombinant GLUT1 antibody.

GLUT1/3132R

IHC-P

Protein G affinity chromatography

Purified

This recombinant GLUT1 antibody is available for research use only.

Store the recombinant GLUT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Optimal dilution of the recombinant GLUT1 antibody should be determined by the researcher.

Cell surface

IHC testing of human tongue with recombinant GLUT1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.