Recombinant GTBP Antilichaam / G/T niet-bindend eiwit / MSH6

MSH6, also called GTBP or G/T mismatch-binding protein, is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H) . It has been suggested that the deficiencies in DNA mismatch repair protein (s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6/GTBP expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI) . MSH6/GTBP always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsible for the MSI.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P52701

Human

A portion of amino acids 1-200 from human G/T mismatch-binding protein was used as the immunogen for the recombinant GTBP antibody.

MSH6/13194R

IHC-P

Protein A affinity

Purified

This recombinant GTBP antibody is available for research use only.

Aliquot the recombinant GTBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant GTBP antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human colon tissue with recombinant GTBP antibody (clone MSH6/13194R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.