Recombinant HCV E2-eiwit

Hepatitis C virus genotype 1 is a core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit and also regulates many host cellular functions such as signaling pathways and apoptosis. It prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Recombinant HCV (Hepatitis C Virus) NS5b protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Polyprotein

E.coli

HCV

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17 kDa (155aa) confirmed by MALDI-TOF

HCV, E2, Polyprotein, ATGP2676-10 µg, ATGP2676-20 µg, ATGP2676-50 µg, ATGP2676-100 µg, ATGP2676-250 µg, ATGP2676-500 µg, ATGP2676-1 mg, ATGP2676-10, ATGP2676-20, ATGP2676-50, ATGP2676-100, ATGP2676-250, ATGP2676-500, ATGP2676-1

Taylor D.R., et al. (2001) J. Virol. 75:1265-1273; ; Kalliampakou K.I., et al. (2015) J. Gen. Virol. 86:1015-1025;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Virus

NP_671491

HCV

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSPMGF SYDTRCFDST VTESDIRTEE AIYQCCDLDP QARVAIKSLT ERLYVGGPLT NSRGENCGYR RCRASGVLTT SCGNTLTCYI KARAACRAAG LQDCTMLVCG DDLVVICESA GVQED