Recombinant HCV E2-eiwit

E1 and E2 glycoproteins form a heterodimer that is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some additional liver specific cofactors may be needed. The fusion function may possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. Recombinant HCV (Hepatitis C Virus) E2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Polyprotein

E.coli

HCV

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

25.4 kDa (226aa)

HCV, HCV E2, Polyprotein, ATGP2758-10 µg, ATGP2758-20 µg, ATGP2758-50 µg, ATGP2758-100 µg, ATGP2758-250 µg, ATGP2758-500 µg, ATGP2758-1 mg, ATGP2758-10, ATGP2758-20, ATGP2758-50, ATGP2758-100, ATGP2758-250, ATGP2758-500, ATGP2758-1

Taylor D.R., et al. (2001) J. Virol. 75:1265-1273; ; Kalliampakou K.I., et al. (2015) J. Gen. Virol. 86:1015-1025;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Virus

NP_671491

HCV

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSERPY CWHYPPRPCG IVPAKSVCGP VYCFTPSPVV VGTTDRSGAP TYSWGANDTD VFVLNNTRPP LGNWFGCTWM NSTGFTKVCG APPCVIGGVG NNTLLCPTDC FRKHPEATYS RCGSGPWITP RCMVDYPYRL WHYPCTINYT IFKVRMYVGG VEHRLEAACN WTRGERCDLE DRDRSELSPL LLSTTQ