Recombinant HCV Kerneiwit

HCV-Core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit and also regulates many host cellular functions such as signaling pathways and apoptosis. HCV-Core prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Recombinant Hepatitis C virus Core protein, fused to His-tag at N-terminus, was expressed in E. coli.

Polyprotein, Human Hepatitis C Virus E2 protein fragment

E.coli

HCV

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.7 kDa (140aa)

HCV, HCV Core, Polyprotein, Human Hepatitis C Virus E2 protein fragment, ATGP2766-10 µg, ATGP2766-20 µg, ATGP2766-50 µg, ATGP2766-100 µg, ATGP2766-250 µg, ATGP2766-500 µg, ATGP2766-1 mg, ATGP2766-10, ATGP2766-20, ATGP2766-50, ATGP2766-100, ATGP2766-250, ATGP2766-500, ATGP2766-1

Taylor D.R., et al. (2001) J. Virol. 75:1265-1273; ; Kalliampakou K.I., et al. (2015) J. Gen. Virol. 86:1015-1025

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Virus

ACE82480

B3TKP0

HCV

MGSSHHHHHH SSGLVPRGSH MSTNPKPQRK TKRNTNRRPQ DVKFPGGGQI VGGVYLLPRR GPRLGVRATR KTSERSQPRG RRQPIPKARR PEGRTWAQPG YPWPLYGNEG CGWAGWLLSP RGSRPSWGPT DPRRRSRNLG