Recombinant HCV NS3-eiwit

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

NS3 protease/helicase, NS3 protease/helicase HCV NS3 transactivated protein, NS 3, NS3P, P70, Serine protease/NTPase/helicase.

E.coli

HCV

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27.9 kDa (268aa) confirmed by MALDI-TOF

HCV, NS3, NS3 protease/helicase, NS3 protease/helicase HCV NS3 transactivated protein, NS 3, NS3P, P70, Serine protease/NTPase/helicase., ATGP0519-10 µg, ATGP0519-20 µg, ATGP0519-50 µg, ATGP0519-100 µg, ATGP0519-250 µg, ATGP0519-500 µg, ATGP0519-1 mg, ATGP0519-10, ATGP0519-20, ATGP0519-50, ATGP0519-100, ATGP0519-250, ATGP0519-500, ATGP0519-1

Hahn JA., et al. (2007) J Infect Dis. 195 (11) :1556-9. ; ; Caruntu FA., et al. (2006) J Gastrointestin Live Dis. 15 (3) :249-56.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Virus

NP_671491

HCV

VAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT