Recombinant HCV NS5a-eiwit

NS5A is a component of the replication complex involved in RNA-binding. Its interaction with Human VAPB may target the viral replication complex to vesicles. This protein down-regulates viral IRES translation initiation and mediates interferon resistance, presumably by interacting with and inhibiting human EIF2AK2/PKR. NS5a seems to inhibit apoptosis by interacting with BIN1 and FKBP8. The hyperphosphorylated form of NS5A is an inhibitor of viral replication. Recombinant HCV (Hepatitis C Virus) NS5A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Polyprotein, Hepatitis C virus non-structural 5a protein membrane anchor

E.coli

HCV

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

13.4 kDa (118aa) confirmed by MALDI-TOF

HCV, HCV NS5a, Polyprotein, Hepatitis C virus non-structural 5a protein membrane anchor, ATGP2739-10 µg, ATGP2739-20 µg, ATGP2739-50 µg, ATGP2739-100 µg, ATGP2739-250 µg, ATGP2739-500 µg, ATGP2739-1 mg, ATGP2739-10, ATGP2739-20, ATGP2739-50, ATGP2739-100, ATGP2739-250, ATGP2739-500, ATGP2739-1

Taylor D.R., et al. (2001) J. Virol. 75:1265-1273; ; Kalliampakou K.I., et al. (2015) J. Gen. Virol. 86:1015-1025

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT

Virus

NP_671491

HCV

MGSSHHHHHH SSGLVPRGSH MSLKATCTAN HDSPDAELIE ANLLWRQEMG GNITRVESENKVVILDSFDP LVAEEDEREV SVPAEILRKS RRFARALPVW ARPDYNPPLV ETWKKPDY