Recombinant Histone H2B (Ubiquityl Lys120) Monoclonaal antilichaam

Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs) . The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human) . This modification triggers a “trans-histone” modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity.

Histone H2B

P62807

Human; Rat; Mouse

Ubiquitinated human histone H2B (Lys120) peptide

HIST1H2BG; H2B.1A; H2B/a; H2BFA; H2BFL; HIST1H2BC; H2BFH; HIST1H2BE; H2BFG; HIST1H2BF; H2BFK; HIST1H2BI; H2BC4; H2BC6; H2BC7; H2BC8; H2BC10

A831

Unconjugated

WB; IHC

Epigenetics and Nuclear Signaling

Protein A purified

1 mg/mL

WB 1:500-1:2000; IHC 1:200-1:1000

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Ice bag

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

https://789.bio/eb/kaVc18Mw

23 kDa

23 kDa

IgG, κ