Recombinant hiv-1 Gag p24 eiwit

The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Group-specific antigen - p24, CA, Capsid protein p24, Group-specific antigen - p24

E.coli

HIV-1

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

21.2 kDa (188aa) confirmed by MALDI-TOF

Gag p24, ATGP0671-10 µg, ATGP0671-20 µg, ATGP0671-50 µg, ATGP0671-100 µg, ATGP0671-250 µg, ATGP0671-500 µg, ATGP0671-1 mg, ATGP0671-10, ATGP0671-20, ATGP0671-50, ATGP0671-100, ATGP0671-250, ATGP0671-500, ATGP0671-1

Bernard NF., et al. (2009) Clin Immunol. 131 (2) :277-87.; ; Wagstaff M., et al. (1995) Immunology. 86 (1) :85-96.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.1mM PMSF, 10% glycerol

Virus

AAA44987.1

P12493

HIV-1

WVKVVEEKA FSPEVIPMFS ALSEGATPQD LNTMLNTVGG HQAAMQMLKE TINEEAAEWD RLHPVHAGPI APGQMREPRG SDIAGTTSTL QEQIGWMTHN PPIPVGEIYK RWIILGLNKI VRMYSPTSIL DIRQGPKEPF RDYVDRFYKT LRAEQASQEV KNWMTETL