Recombinant humaan 14-3-3 bèta/alfa eiwit

YWHAB, also known as 14-3-3 protein beta/alpha, is 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human YWHAB, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

YWHAB, YWHAA, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha N-terminally processed, GW128, HEL-S-1, HS1, KCIP-1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

30.6 kDa (270aa) confirmed by MALDI-TOF

1433 b, 1433 beta, 14-3-3 protein beta/alpha, GW128, HEL-S-1, HS1, KCIP-1, YWHAA, ATGP3590-10 µg, ATGP3590-20 µg, ATGP3590-50 µg, ATGP3590-100 µg, ATGP3590-250 µg, ATGP3590-500 µg, ATGP3590-1 mg, ATGP3590-10, ATGP3590-20, ATGP3590-50, ATGP3590-100, ATGP3590-250, ATGP3590-500, ATGP3590-1

Rodriguez LG., et al. (2005) J Cell Physiol. 202 (1) :285-94.; ; Mils V., et al. (2000) Oncogene. 19 (10) :1257-65.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_003395

P31946

Human

MGSSHHHHHH SSGLVPRGSH MGSHMTMDKS ELVQKAKLAE QAERYDDMAA AMKAVTEQGH ELSNEERNLL SVAYKNVVGA RRSSWRVISS IEQKTERNEK KQQMGKEYRE KIEAELQDIC NDVLELLDKY LIPNATQPES KVFYLKMKGD YFRYLSEVAS GDNKQTTVSN SQQAYQEAFE ISKKEMQPTH PIRLGLALNF SVFYYEILNS PEKACSLAKT AFDEAIAELD TLNEESYKDS TLIMQLLRDN LTLWTSENQG DEGDAGEGEN