Recombinant humaan 14-3-3 sigma-eiwit

14-3-3 sigma, also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E. coli and purified by using conventional chromatography techniques.

Stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27.7 kDa (248aa) confirmed by MALDI-TOF

1433 sigma, stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma, ATGP0435-10 µg, ATGP0435-20 µg, ATGP0435-50 µg, ATGP0435-100 µg, ATGP0435-250 µg, ATGP0435-500 µg, ATGP0435-1 mg, ATGP0435-10, ATGP0435-20, ATGP0435-50, ATGP0435-100, ATGP0435-250, ATGP0435-500, ATGP0435-1

Benzinger A., et al. (2005) Mol Cell Proteomics. 4 (6) : 785-95.; ; Wilker E W., et al. (2005) J Biol Chem. 280:18891-18898.;

Arakawa N, et al. Serum stratifin and presepsin as candidate biomarkers for early detection of COVID-19 disease progression. (J Pharmacol Sci. 2022) {https://pubmed.ncbi.nlm.nih.gov/35926945/}; ; Arakawa N, et al. Stratifin as a novel diagnostic biomarker in serum for diffuse alveolar damage. (Nat Commun. 2022) {https://pubmed.ncbi.nlm.nih.gov/36195613/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl, 10% glycerol

Apoptosis

NP_006133

P31947

Human

MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS