Recombinant humaan 15-PGDH/HPGD-eiwit

HPGD, also known as 15-PGDH, is the fundamental enzyme of prostaglandin degradation. This protein strongly reduces the biologic activity of these molecules by catalyzing the oxidation of the 15-hydroxyl group of prostaglandins to a keto group. HPGD is implicated in many physiologic and cellular processes, such as inflammation. Recombinant human HPGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

15-hydroxyprostaglandin dehydrogenase [NAD+], 15-PGDH, PGDH, PGDH1, SDR36C1, Prostaglandin dehydrogenase 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.1 (286aa) confirmed by MALDI-TOF

15 Hydroxyprostaglandin dehydrogenase, 15 Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15 hydroxyprostaglandin dehydrogenase [NAD+], 15 PGDH, 15-Hydroxyprostaglandin dehydrogenase, 15-Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15-hydroxyprostaglandin dehydrogenase [NAD+], 15PGDH, 15-PGDH, AV026552, HPGD, PGDH, PGDH 1, PGDH1, PHOAR 1, PHOAR1, Prostaglandin dehydrogenase 1, Prostaglandin dehydrogenase1, SDR36C 1, SDR36C1, ATGP0719-10 µg, ATGP0719-20 µg, ATGP0719-50 µg, ATGP0719-100 µg, ATGP0719-250 µg, ATGP0719-500 µg, ATGP0719-1 mg, ATGP0719-010, ATGP0719-020, ATGP0719-050, ATGP0719-100, ATGP0719-250, ATGP0719-500, ATGP0719-01M

Han X., et al. (1995) Equine Vet. J. 27: 334-339.; ; Van Meir C.A., et al. (1996) Placenta 17: 291-297.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Immunology

NP_000851

P15428

Human

MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM GQYIEYKDHI KDMIKYYGIL DPPLIANGLI TLIEDDALNG AIMKITTSKG IHFQDYDTTP FQAKTQ