Recombinant humaan 17 bèta-HSD10/HSD17B10-eiwit

HSD17B10 is a member of the short-chain dehydrogenase/reductase superfamily. This mitochondrial protein catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. HSD17B10 plays an important role in processing steroid hormones and fats, and also helps break down the protein building block (amino acid) isoleucine. This enzyme is also necessary for several chemical reactions involving female sex hormones (estrogens) and male sex hormones (androgens) . It is essential for maintaining appropriate levels of male and female sex hormones. This protein may contribute to the neuronal dysfunction associated with Alzheimer disease. Recombinant HSD17B10 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Hydroxysteroid 17-beta dehydrogenase 10, HADH2, MRXS10, ERAB, SCHAD, MHBD, 17b-HSD10, ABAD, SDR5C1, MRPP2, CAMR, Hydroxyacyl-Coenzyme A dehydrogenase, type II, mental retardation, X-linked, syndromic 10, Hydroxysteroid (17-beta) dehydrogenase 10, type 10 17b-HSD, type 10 17beta-hydroxysteroid dehydrogenase, AB-binding alcohol dehydrogenase, short chain dehydrogenase/reductase family 5C, member 1, mitochondrial RNase P subunit 2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.1 kDa (271aa) confirmed by MALDI-TOF

HSD17B10, 3-hydroxyacyl-CoA dehydrogenase type-2, Hydroxyacyl Coenzyme A dehydrogenase type II, Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase, 17 beta hydroxysteroid dehydrogenase 10, 17 beta hydroxysteroid dehydrogenase type 10, 17b HSD10, 3 hydroxy 2 methylbutyryl CoA dehydrogenase, 3 hydroxyacyl CoA dehydrogenase type 2, 3 hydroxyacyl CoA dehydrogenase type II, AB binding alcohol dehydrogenase, ABAD, Ads9, Amyloid beta binding polypeptide, Amyloid beta peptide binding alcohol dehydrogenase, Amyloid beta peptide binding protein, CAMR, DuPXp11.22, Endoplasmic Reticulum Amyloid Binding Protein, Endoplasmic reticulum associated amyloid beta peptide binding protein, ER associated amyloid beta-binding protein, ERAB, HADH 2, HADH2, HCD 2, HCD2, HSD17B10, Hydroxyacyl CoA Dehydrogenase type II, Hydroxysteroid (17 beta) dehydrogenase 10, Mental retardation X linked syndromic 11, MHBD, Mitochondrial ribonuclease P protein 2, Mitochondrial RNase P protein 2, MRPP2, MRX17, SCHAD, SDR5C1, Short

Hoizmann J., et al. (2008) Cell. 135 (3) :462-74. ; ; Yan SD., et al. (1997) Nature. 389 (6652) :689-95. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, and 100 mM NaCl.

Redox Proteins

NP_004484.1

Q99714

Human

MVAVITGGAS GLGLATAERL VGQGASAVLL DLPNSGGEAQ AKKLGNNCVF APADVTSEKD VQTALALAKG KFGRVDVAVN CAGIAVASKT YNLKKGQTHT LEDFQRVLDV NLMGTFNVIR LVAGEMGQNE PDQGGQRGVI INTASVAAFE GQVGQAAYSA SKGGIVGMTL PIARDLAPIG IRVMTIAPGL FGTPLLTSLP EKVCNFLASQ VPFPSRLGDP AEYAHLVQAI IENPFLNGEV IRLDGAIRMQ P