Recombinant humaan 17 bèta-HSD11/HSD17B11 eiwit

HSD17B11, also known as DHRS8 (dehydrogenase/reductase SDR family member 8), belongs to the HSD17B family of proteins, which regulate the availability of steroids within various tissues throughout the body. It is widely expressed with highest levels found in retina, pancreas, kidney, liver, lung, adrenal, small intestine, ovary and heart as well as in steroidogenic cells. This protein converts androstan-3-alpha, 17-beta-diol (3-alpha-diol) to androsterone, suggesting it may participate in androgen metabolism during steroidogenesis. Recombinant human HSD17B11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hydroxysteroid 17-beta dehydrogenase 11, Estradiol 17-beta-dehydrogenase 11, DHRS8, PAN1B, SDR16C2, 17-beta-hydroxysteroid dehydrogenase 11. 17-beta-HSD 11, 17bHSD11, 17betaHSD11, 17-beta-hydroxysteroid dehydrogenase XI, 17-beta-HSD XI, 17betaHSDXI, Cutaneous T-cell lymphoma-associated antigen HD-CL-03, CTCL-associated antigen HD-CL-03, Dehydrogenase/reductase SDR family member 8, Retinal short-chain dehydrogenase/reductase 2, retSDR2, Short chain dehydrogenase/reductase family 16C member 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.4 kDa (287aa) confirmed by MALDI-TOF

17 beta HSD 11, 17 beta HSD XI, 17 beta hydroxysteroid dehydrogenase 11, 17 beta hydroxysteroid dehydrogenase XI, 17-beta-HSD 11, 17-beta-HSD XI, 17betaHSD11, 17betaHSDXI, 17-beta-hydroxysteroid dehydrogenase 11. 17-beta-HSD 11, 17-beta-hydroxysteroid dehydrogenase XI, 17BHSD11, CTCL associated antigen HD CL 03, CTCL associated antigen HD-CL-03, CTCL-associated antigen HD-CL-03, Cutaneous T cell lymphoma-associated antigen HD CL 03, Cutaneous T cell lymphoma-associated antigen HD-CL-03, Cutaneous T-cell lymphoma-associated antigen HD CL 03, Cutaneous T-cell lymphoma-associated antigen HD-CL-03, Dehydrogenase/reductase SDR family member 8, DHRS8, Estradiol 17 beta dehydrogenase 11, Estradiol 17-beta-dehydrogenase 11, HSD17B11, Hydroxysteroid (17-beta) dehydrogenase 11, Hydroxysteroid 17 beta dehydrogenase 11, Hydroxysteroid 17-beta dehydrogenase 11, PAN1B, Retinal short chain dehydrogenase/reductase 2, Retinal short-chain dehydrogenase/reductase 2, RETSDR2, SDR16C2, Short chain dehydrog

Rotinen M., et al. (2010) J Steroid Biochem Mol Biol. 122 (4) :164-71.; ; Nakamura Y., et al. (2009) Neoplasma. 56 (4) :317-20.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Enzymes & Proteases

NP_057329

Q8NBQ5

Human

MGSSHHHHHH SSGLVPRGSH MESFVKLFIP KRRKSVTGEI VLITGAGHGI GRLTAYEFAK LKSKLVLWDI NKHGLEETAA KCKGLGAKVH TFVVDCSNRE DIYSSAKKVK AEIGDVSILV NNAGVVYTSD LFATQDPQIE KTFEVNVLAH FWTTKAFLPA MTKNNHGHIV TVASAAGHVS VPFLLAYCSS KFAAVGFHKT LTDELAALQI TGVKTTCLCP NFVNTGFIKN PSTSLGPTLE PEEVVNRLMH GILTEQKMIF IPSSIAFLTT LERILPERFL AVLKQKI